Naslov
Isolation and identification of fowl Poxvirus using PCR applied as a vaccine by nebulisation to newly-hatched chickens

Autori
Gottstein, Željko ; Mazija, Hrvoje ; Zuckermann-Šoštarić, Ivan Conrado

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
World's Poultry Science Journal / Romboli, I. ; Flock, D. ; Franchini, A. - Beekbergen : Personal Media Solutions B.V., 2006

Skup
XII European Poultry Conference

Mjesto i datum
Verona, Italija, 10.09.2006. - 14.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chickens; Poxvirus; vaccination; nebulisation; PCR

Sažetak
Newly-hatched chickens vaccinated with commercial fowl Poxvirus vaccine (BODIKAL&reg ; SPF, Vetrina d.o.o., Zagreb, Croatia) delivered by nebulisation did not show any clinical reaction to the given vaccine compared to negative (nonvaccinated) and positive (vaccinated by wing web)controls. To detect location and spread of vaccine virus, chickens were sacrificed and tissues were taken. Using commercially available kits, whole DNA was isolated from the tissue samples and viral DNA was detected using two different specific primer pairs in PCR reaction. Viral DNA was detected in trachea of chickens 20 hours and in lungs 7 and 14 days after vaccination by nebulisation. In positive controls, vaccinated by wing web method, viral DNA was detected at the site of vaccination in wing web tissue, and bone marrow on day 7 after vaccination, but none was detected in any other tissue of chickens in vaccinated groups during the trial. To define protection level after vaccination chickens were challenged into the wing web using velogenic field strain and skin reaction was measured and analyzed statistically. The size of the skin reaction in nebulised and wing web vaccinated chickens was significantly smaller compared to the negative control five days after challenge (day 26).

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA