Naslov
Electrochemical methods for quantification and characterization of metallothioneins induced in Mytilus galloprovincialis

Autori
Raspor, Biserka ; Pavičić, Jasenka

Izvornik
Fresenius journal of analytical chemistry (0937-0633) 354 (1996), 5-6; 529-534

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
metallothioneins ; quantification of ; characterization of

Sažetak
The quantification of the purified metallothionein (MT) component, isolated from the digestive gland of cadmium-exposed Mytilus galloprovincialis , is described based on the analysis of Cd(II) and SH-groups content, applying electrochemical methods. Advantages and disadvantages of the Brdi~ka procedure for the determination of the MT content is discussed. The saturation of binding positions of purified MT with Cd2+ ions can be directly followed voltammetrically. Irrespective of the MT concentration, the saturation with Cd2+ of in vivo induced mussel MT is achieved at a molar ratio of 5. Cd2+ ions are rapidly displaced from the Cd-Th complex after the addition of Pb2+ ions, which indicates the kinetically labile type of the complex.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

Napomena
Conference: 6th International Hans Wolfgang Nurnberg Memorial Symposium on Metal Compounds in Environment and Life Location: FORSCHUNGSZENTRUM JULICH, JULICH, GERMANY Date: MAY 09-12, 1995

POVEZANOST RADA