Naslov
Modification of the giemsa-staining method for mitotic chromosomes of phytopathogenic fungus Botrytis cinerea Pers. ex Fr.

Autori
Halupecki, Edyta ; Topolovec-Pintarić Snježana ; Bogdan, Cvjetković

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum / Vitale, Branko - Zagreb : Periodicum biologorum, Hrvatsko prirodoslovno društvo, 1998, 47-47

Skup
First Congress of Croatian Geneticists with International Participation

Mjesto i datum
Hvar, Hrvatska, 01.06.1998. - 04.06.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Botrytis cinerea; Giemsa-staining; chromosomes

Sažetak
Only a few cytogenetical studies on Botrytis cinerea, an economically important phytopathogenic fungus of well known morphology and epidemiology, were conducted until today. The successful attempts in visualisation of mitotic chromosomes are rare, probably because of the small size of vegetative hifa and chromosomes, but also due to the specific process of mitosis (Shirane et al., 1988, 1989 ; Taga i Murata, 1994). Difficulties in observation of mitotic chromosomes are those concerning the nuclear membrane which remains intact throughout division of the nucleus, and, also, presence of the rigid cell wall which affects efficiency of chromosome staining. Shirane et al. (1988) overcome these physical barriers using germlings of conidia by bursting their tips with methanol-acetic acid treatment, which resulted in successful releasing of metaphase chromosomes. Our modification of Shirane's method significantly shortened time required to perform this chromosome-staining technique without reducing quality of result.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA