Naslov
Flow cytometric determination of the leukocyte subsets in paraffinstimulated whole human saliva

Autori
Vidović, Anđelko ; Vidović Juras, Danica ; Cekić-Arambašin, Ana ; Lukač, Josip ; Sabioncello, Ante ; Dekaris, Dragan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Oral Diseases. Volume 12 (S1) P63 / - , 2006, 24-25

Skup
Biennial Congress of the European Association of Oral Medicine (8 ; 2006)

Mjesto i datum
Zagreb, Hrvatska, 31.08.2006. - 02.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
flow cytometry; saliva; leukocyte subsets

Sažetak
Human saliva has an important role in the oral immune system which is a part of an extensive and specialized mucosa-associated lymphoid tissue. Antimicrobial properties of saliva were primarily investigated through its soluble components and little is known about its cellular components. We developed a method to quantify leukocytes in paraffin-stimulated whole human saliva (SWS) using four color flow cytometry and performed reliability study on 8 healthy volunteers (4 males, 4 females, mean age 26.1, SD 1.1). Based on the stains we used (CD45 FITC, CD14 PE, CD20 PerCP, CD3APC)and FITC threshold gating, we could discriminate major leukocyte populations(granulocytes, monocytes and lymphocytes), as well as T and B cells, and measure their percentages. Saliva values were compared to the whole blood values analyzed according to standard laboratory procedure. Predominant leukocyte population in saliva were granulocytes (97.5%, SD 2.1) followed by very low frequencies of monocytes (1.3%, SD 0.7) and lymphocytes (1.2%, SD 1.5). Percentages of T (72.32%, SD 9.23) or B (11.87%, SD 6.33) cells in saliva did not differ significantly from the peripheral blood values. Intra-class coefficients of correlation (ICC) of assessed parameters ranged from 0.6 to 0.9 suggesting generally good reliability of the measurement. Flow cytometry can be used to reliably quantify leukocytes and its subsets in paraffin SWS. The identification and complete characterization of salivary leukocytes could be used to better understand pathogenesis of many oral diseases.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

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