Naslov
Overexpression of Phenylalanine ammonia-lyase and Rosmarinic Acid Production in Transgenic Roots of Coleus blumei Benth.

Autori
Bauer, Natasa ; Fulgosi, Hrvoje ; Leljak-Levanić, Dunja ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
11th IAPTC&B Congress, Biotechnology and Sustainable Agriculture 2006 and Beyond, Abstracts / Pua, Eng-Chong - Peking, 2006

Skup
11th IAPTC&B Congress, Biotechnology and Sustainable Agriculture 2006 and Beyond

Mjesto i datum
Peking, Kina, 13.08.2006. - 18.08.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ukrasna kopriva; ružmarinska kiselina; transformacija; agrobakterije; fenilalanin-amonij lijaza
(Coleus blumei; rosmarinic acid; transformation; Agrobacterium; phenylalanine ammonia-lyase)

Sažetak
Rosmarinic acid (RA) is common caffeic acid ester in the plant kingdom. In plants it acts as a preformed constitutively accumulated defense compound. It is biosynthesized via the phenyl-propanoid pathway. The eight enzymes involved in the biosynthesis are known and characterized. Precursor for the caffeic acid moiety of RA is L-phenylalanine. Phenylalanine ammonia-lyase (PAL) catalyzes the oxidative desamination of L-phenylalanine. Previous results indicate that induction of PAL activity is the consequence of transcriptional activation of PAL genes. The correlation of the PAL activity with RA synthesis is noticed. To get more knowledge on that correlation, activity of phenylalanine ammonia-lyase, in transgenic roots of Coleus blumei Benth. was perturbed by introduction of an Arabidopsis thaliana PAL1 gene controlled by the CaMV 35S promoter. Thirteen transgenic root lines resistant to gluphosinate ammonium (selective marker) were established and maintained on MS medium supplemented with 5.4  M NAA. The content of RA in root cultures was quantified by HPLC, and PAL activity was measured spectrophotometricaly on 300 nm with L-phenylalanine as a substrate. Comparing with control-normal root line, all 13 transgenic lines had strong enhancement of PAL activity that was reflected on induction of phenolic production. Rosmarinic acid accumulation was significantly induce in 8 lines. In one transgenic root line RA production was diminished and greater amount of chlorogenic acid was noticed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA