Naslov
MATURE CD83+ DENDRITIC CELLS ACTIVATE DECIDUAL NK CELLS

Autori
Laskarin, Gordana ; Strbo, Natasa ; Juretic Frankovic, Koraljka ; Dupor, Jana ; Veljkovic, Danijela ; Haller, Herman ; Stimac, Tea ; Allavena, Paola ; Mantovani, Aleberto ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book Molecular Mechanisms of Implantation, Second EMBIC Summer School / Szekeres-Bartho, Julia - Pečuh : Sveučilište u Pećuhu, 2006, 18-18

Skup
The Second EMBIC Summer School: Molecular Mechanisms of Implantation

Mjesto i datum
Pečuh, Mađarska, 01.07.2006. - 04.07.2006

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dendritic cells; NK cells; Pregnancy

Sažetak
Problem: The influence of decidual dendritic cells (DC) on decidual NK cells was investigated. Material and methods: Distribution of CD83+ cells in early pregnancy decidua was analysed in comparison with trophoblast invasion and NK cell-infiltration using immunohistology. Phenotype, origin, maturation state and cytokine expression of DC were detected by flow cytometry. Magnetically purified CD56+ and CD83+ cells were co-cultured. Proliferation CFSE assay, CD69 and cytolytic mediators expression in NK cells were analysed. Results: CD83+DC were found in the vicinity of NK cells at the area of trophoblast invasion and expressed CD86, HLA-DR and CD11c in vitro. DC expressed also IL-15, IL-18 and IFNgamma. CD83+DC increased proliferation, CD69 and cytolytic mediator expression in decidual NK cells. Conclusion: Mature decidual DC of mostly myeloid origin are capable to activate decidual NK cells in vitro. The investigation was supported by Croatian Ministry of Science, Grant No. 0062029 and European FVI Project (EMBIC, LSHM-CT-2004-512040, NoE).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA