Naslov
Pulmonary toxicity of Stachybotrys chartarum in experiment

Autori
Kovacikova, Zuzana ; Pieckova, Elena ; Tatrai, Erzsebet ; Hurbankova, Marta ; Pivovarova, Zuzana ; Cerna, Silvia ; Mataušić-Pišl, Mirjana ; Tulinska, Jana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology Letters / Kniewald, J. - Amsterdam : Elsevier, 2006, 16-16

ISBN
2-904938-02-8

Skup
The EUROTOX 2006/6 CTDC Congress

Mjesto i datum
Cavtat, Hrvatska, 20.09.2006. - 24.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
epithelial type II cells; bronchoalveolar lavage; endometabolites

Sažetak
Microbial growth affects indoor air quality and concerned occupants and workers often report respiratory symptoms. Our experiments were focused on Stachybotrys chartarum what has been linked to chronic intoxications, allergies, especially in babies. Stachybotrys chartarum produce several different metabolites with different effects, some of them can act synergistically. They may represent a potential but still poorly documented hazard. During study of indoor fungal colonization in Slovakia also several cases of S. chartarum were found. Isolates were cultivated and tested for their ability to produce toxic metabolites (endometabolites – concentrated in the fungus and exometabolites – released to the cultivation media). The extracts of both were tested in vivo in rats and in vitro in lung cells isolated from rats and organ cultures from chick tracheas. In vivo experiment: Wistar rats were intratrachealy exposed for 3 days to 4 μ g of metabolites in 0.2% DMSO. After finishing the exposure the bronchoalveolar lavage was performed. It revealed lowered viability of alveolar macrophages and enhanced activity of lysosomal enzymes in exposed groups in comparison to the control one. In vitro experiments: alveolar macrophages and lung epithelial type II cells were isolated from rat lung and cultivated in the presence of various concentrations of metabolites. After 24 h cultivation the activity of acid phosphatase in alveolar macrophages was decreased, the production of MCP-1 and TNF-α by both cell types was changed, the number of alkaline positive type II cells was decreased and the histochemical staining with Maclura pomifera showed changes of type II cell membranes. All differences were dose-dependent. The ciliostatic activity of metabolites was followed for 3 days in organ culture from chick trachea. The effect was time- and dose-dependent and the toxicity of exometabolites was higher. The toxic effects of metabolites isolated from Stachybotrys chartarum were proved in all experimental models used in our experiments

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA