Naslov
Effet de glucides métabolisables et non métabolisables sur la croissance, la différenciation cellulaire et l'expression de protéines cellulaires et pariétales de lignées de betterave sucriè re normale et habituées
(Effect of metabolizable and non-metabolizable carbohydrates on growth, cellular differentiation and cellular and extracellular protein expression in normal and habituated sugar beet lines)

Autori
Pavoković, Dubravko

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Faculte des Sciences

Mjesto
Orléans, France

Datum
09.12

Godina
2005

Stranica
209

Mentor
Krsnik-Rasol, Marijana

Neposredni voditelj
Hagege, Daniel

Ključne riječi
Differentiation; sugar sensing; sugar beet; signal transduction; proteomic; glycoproteins; 2-D electrophoresis

Sažetak
Carbohydrates play important roles in plants, by changing gene expression and developmental patterns. Three sugar beet cell lines (Beta vulgaris L.) N, HO and HNO on different stages of development were used in this study. Our objective was to establish a relationship between possible deviations in sugar perception/signalling process and differentiation state of the cell lines. We have grown cell lines on medium with different carbohydrates, measured activities of sucrose metabolism, tested sugar perception and signalling with sugars, sugar analogs and signal transduction inhibitors. The effect of sugars on protein and glycoprotein pattern was examined by electrophoretic separation of proteins, characterization of glycan component of glycoproteins and protein identification by mass spectrometry. Collective results show differences in utilization of carbohydrates from solid medium, and in activities of sucrose metabolism. The regulation of these enzymes was sugar dependent but differed among the cell lines. Signal perception was different among the cell lines but the part of the signal transduction pathways that involved phosphorylation/dephosphorylation events was conserved. The SDS-PAGE and 2-D electrophoretic analysis revealed sugar regulated proteins. Protein and glycoprotein pattern of extracellular proteins was sugar regulated. Sialic acid was characterized as a glycan component of extracellular proteins. Using mass spectrometry, endochitinase SP2 was identified as extracellular protein with differential expression in cell lines.

Izvorni jezik
Fra-eng

Znanstvena područja
Biologija

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