Naslov
RAST, POTROŠNJA GLUKOZE I EKSKRECIJA PROTEINA U TRI EMBRIOGENE LINJE BUNDEVE (Cucurbita pepo L.)
(GROWTH PARAMETERS, GLUCOSE UPTAKE AND PROTEIN EXCRETION OF THREE EMBRYOGENIC LINES OF PUMPKIN (Cucurbita pepo L.))

Autori
Mihaljević, Snježana ; Garić, Rade ; Horvat, Gordana ; Leljak-Levanić, Dunja ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of Abstracts (Zbornik sažetaka), 9th Croatian Biological Congress / Besendorfer, Višnja ; Filipović, Indira - Zagreb : Hrvatsko biološko društvo, 2006, 184-185

Skup
9th Croatian Biological Congress (9. Hrvatski biološki kongress)

Mjesto i datum
Rovinj, Hrvatska, 23.09.2006. - 29.09.2006

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
dušik; pH; izlučivanje proteina; somatska embriogeneza
(nitrogen; pH; protein excretion; somatic embryogenesis)

Sažetak
The effects of medium composition on the growth, metabolism and protein excretion of three embryogenic lines grown in liquid medium were studied as a step towards elucidating the role of nitrogen supply in the regulation of developmental transition of pumpkin somatic embryos. All parameters were measured over a 15-day culture period on days 0, 4, 8, 11 and 15. Fresh and dry weights were compared with the glucose uptake and pH of the culture media in order to assess growth kinetics. Ammonium, used as the sole source of nitrogen, negatively influenced embryogenic tissue growth and morphogenesis and caused a significant decrease in the media pH. Buffering ammonium-only medium with MES stabilized the pH, enhanced tissue proliferation and facilitated protein excretion. Globular embryos were formed at a low frequency and no further differentiation into late-stage embryos was observed. Maturation of embryos proceeded only when a combination of reduced (NH4+) and unreduced (NO3-) forms of nitrogen was provided in the medium. A noticeable effect of NO3- re-supply of cultures previously maintained on the NH4+- containing medium reflected through increased growth, glucose uptake and protein excretion in the culture medium. A comparative analysis of extracellular proteins in pumpkin embryogenic tissue lines was performed with the intention to detect some nutrient-dependent changes related to the stage of development.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Biologija

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