Naslov
Effects of in vitro cadmium exposure on ovarian steroidogenesis in rats

Autori
Piasek, Martina ; Laskey, John W.

Izvornik
Journal of applied toxicology (0260-437X) 19 (1999), 3; 211-217

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cadmium; estrus cycle; ovarian culture; pregnancy; rat; specific radioimunoassay; steroid hormone production

Sažetak
The purpose of the study was to evaluate the direct effect(s) of in vitro cadmium (Cd) exposure on steroidogenesis in rat ovaries during different reproductive states. Sprague-Dawley rats were killed on the day of proestrus, or on gestation day 6 or 16. Ovaries were removed, placed in medium and minced. Culture from each ovary was incubated with Cd(2+) ions in concentrations of 0, 100, 500, 1000, 1500, or 2000 ľM. One-hour whole ovary production of progesterone, testosterone and estradiol in culture medium was evaluated in absence and presence of hCG or hCG plus pregnenolone by specific radioimmunoassay. Under in vitro Cd exposure the most affected were productions of progesterone and testosterone in proestrus rats and less in pregnant dams, while estradiol was unaffected at all. Cadmium appears to interfere with the ovarian steroidogenic pathway in rats at more than one site.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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