Naslov
Experimental Models of Demyelination

Autori
Barac-Latas, Vesna ; Radošević-Stašić, Biserka ; Sepčić, Juraj ; Rukavina, Daniel

Izvornik
Acta Clinica Croatica (0353-9466) 36 (1997); 58-59

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
subacute demyelinating encephalomyelitis; MHV-JHM coronavirus; apoptosis; necrosis

Sažetak
Results derived from neuropathologic findings in Lewis rats with developed subacute demyelinating encephalomyelitis SDE indicated the existence of three developmental stages of the disease, that mutually differ according to the virus-infected cell count, inflammatory cell count, grade of myelin damage and functionaly viable PLP mRNA cell count. The first stage of SDE was found to be characterized by extensive viral infection of the white matter of the brain and spinal cord, accompanied by inflammatory reaction. There were no sings of myelin and tissue damage, but the synthesis of myelin was reduced already at this stage, expressed as a decrease in the PLP mRNA cell count. In the second stage of the disease, sharply delineated demyelination areas, accompanied by strong inflammatory reaction, were observed. Beside the loss of a great number of oligodendrocytes, a loss of astrocytes was also recorded. There was pronounced gliosos on lesion margins. The number of virus-infected cells that remained in the area close to the lesion decreased. A great proportion of cells showed signs of degeneration, necrosis or apoptosis. At this stage of development, even some areas of remyelination occured at an interesting and unusual localization, i.e. in the center of the demyelinating lesion. In the third stage of the disease, the virus almost disappeared. The tissue was severely damaged, occasionally nacrotic. Active demyelination and massive macrophage infiltration could still be observed. A large proportion of cells showed signs of apoptosis. Among the cells that exhibited the signs of apoptosis, T-lymphocytes and oligodendrocytes were most frequently isolated in all the three developmental stages of the disease. Among oligodendrocytes, the virus-infected cells underwent necrosis, whereas noninfected cells showed the signs of apoptosis. Like in autoimmune encephalomyelitis, in coronavirus-induced encephalomyelitis the inflammatory cells, T-lymphocytes, are also cleared from CNS by the process of apoptosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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