Naslov
DNA polymorphisms in Austrian and Dalmatian black pine

Autori
Liber, Zlatko ; Pavletić, Zinka ; Nikolić, Toni ; Fulgosi, Hrvoje

Izvornik
Wissenschaftliche Mitteilungen aus dem Niederösterreichischen Landmuseum 12 (1999) 53-62 ; ISSN 0506-919X

Vrsta, podvrsta
Ostale vrste radova, ostali članci/prilozi

Godina
1999

Ključne riječi
black pine; cpDNA; DNA polymorphisms; Pinus nigra; RAPD; RFLP; molecular systematics

Sažetak
The systematic status of Dalmatian black pine is still unexplained. This black pine is usually considered as subspecies but sometimes also as a separate species. Austrian black pine from Botanical Garden Zagreb, Dalmatian black pine from the mount of Biokovo and Dalmatian black pine from the island of Hvar were used in this study. Two methods of plant molecular systematics have been applied in this work: restriction fragment length polymorphisms of chloroplast DNA (RFLP cpDNA) and random amplified polymorphic DNA (RAPD). The results of RFLP analysis of chloroplast DNA have been showed that there is no differences in restriction fragments among all investigated plants. The RAPD method which is the most appropriate for plant systematic at the species level and below, has been shown DNA polymorphisms between and within investigated populations, distinctly separated Austrian and Dalmatian black pine. The subspecies level for Dalmatian black pine has been strongly supported by these methods.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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