Naslov
DNA repair in Deinococcus radiodurans: from hundreds of fragments to the functional chromosome

Autori
Zahradka, Ksenija ; Radman, Miroslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka 9. hrvatskog biološkog kongresa s međunarodnim sudjelovanjem / Besendorfer, Višnja ; Klobučar, Goran - Zagreb : Hrvatsko biološko društvo, 2006, 59-60

Skup
9. hrvatski biološki kongres s međunarodnim sudjelovanjem

Mjesto i datum
Rovinj, Hrvatska, 23.09.2006. - 29.09.2006

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Deinococcus radiodurans; DNA repair; homologous recombination; DNA synthesis

Sažetak
Bacterium Deinococcus radiodurans is one of the most radiation resistant organisms known. It can sustain extreme levels of ionizing radiation breaking its genome into hundreds of shorts fragments. Remarkably, in just couple of hours these fragments are reassembled into functional chromosomes due to an efficient and precise DNA repair process. We found that this repair involves a special kind of &laquo ; ; extended synthesis-dependent strand annealing&raquo ; ; (ESDSA) followed by homologous recombination. In ESDSA, chromosomal fragments produced by radiation are used both as primers and templates for a massive synthesis of long single strand extensions which then anneal thereby accurately connecting contigous DNA fragments into long linear intermediates. These intermediates are finally matured by RecA-dependent homologous recombination into functional circular chromosomes that are patchworks of old (synthesized before radiation) and new (synthesized after radiation) double-stranded DNA blocks.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA