Naslov
PMA and doxorubicin decrease viability, MTT activity, and expression of CD10 marker on NALM-1 leukemic cells.

Autori
Martin-Kleiner, Irena ; Svoboda-Beusan, Ivna ; Gabrilovac, Jelka

Izvornik
Immunopharmacology and Immunotoxicology (0892-3973) 28 (2006); 411-420

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
pre-B leukemic cells; CD10; NALM-1; PMA; doxorubicin

Sažetak
PMA 10, 20 ng/ml) and doxorubicin (5-20 ng/ml) decreased the viability and MTT-activity of NALM-1 pre-B leukemic cells (3 days treatment). Further, CD10 was downregulated, suggesting that PMA and doxorubicin induced differentiation of NALM-1 cells. However, PMA did not alter expression of B cell markers CD20 and of mIgM. In contrast to PMA, another differentiation agent ATRA did not alter CD10 expression on NALM-1 cells but affected viability after 6 days (5, 10 ng/ml). The data on this study are the first evidence that PMA and doxorubicin inhibited viability and MTT activity and induced partial differentiation, by decreasing CD10 on NALM-1 cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA