Naslov
The role of the RecC subunit of the RecBCD enzyme in recombination and DNA repair

Autori
Vlašić, Ignacija ; Ivančić-Baće, Ivana ; Salaj-Šmic, Erika ; Jeličić, Branka ; Sopta, Mary ; Brčić-Kostić, Krunoslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 1st MedILS Summer School, Structure and Evolution : from Bench to Terminal / - Split : Mediterranean Institute for Life Sciences, 2006, 43-43

Skup
MedILS Summer School, Structure and Evolution : from Bench to Terminal (1 ; 2006)

Mjesto i datum
Split, Hrvatska, 18.07.2006. - 22.07.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
RecC subunit; RecBCD enzyme; recombination; DNA repair

Sažetak
The crystal structure of the RecBCD enzyme has been recently solved, which enables more precise research of the least known RecC subunit. It is already known that the N-terminal domain of the RecC subunit is required for Chi activity while the C-terminal domain is important for RecD assembly in RecBCD. To gain further insight into the importance of the RecC subunit several recC mutants have been constructed and characterized. Typical nonsense or missense mutants in the C-terminal domain of the recC gene are recombination proficient but nuclease deficient, a phenotype which mimics that of recD deletion mutations. In this study, we focused on the previously characterized recC73 mutant that has a nonfunctional C-terminal domain (deletion of T on 1938 position). In addition, we also made five new point-missense recC mutants using PCR site directed mutagenesis. According to the literature, the recC73 mutant is considered a null mutant. In contrast, we showed that the recC73 mutant is recombination proficient but only when some of the gene products from the RecF pathway are present (RecFOR and RecJ). The characterization of five additional missense recC mutants is underway.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA