Naslov
Histopathological effects of PCP on the kidney of freshwater snail Planorbarius corneus measured by using automated image analysis.
(Histopathological effects of PCP on the kidney of freshwater snail Planorbarius corneus measured by using automated image analysis. Proceedings of SECOTOX 99, Munich, Germany, P G5.)

Autori
Klobučar, Goran I.V. ; Lajtner, Jasna ; Erben, Radovan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of SECOTOX 99, Munich, Njemačka, 65. / Kettrup, A. ; Schramm K.W. - München : GSF-Forschungszentrum fur Umwelt und Gesundhait, GmbH, 1999

Skup
Fifth European Conference on Ecotoxicology and Environmental Safety - SECOTOX 99

Mjesto i datum
München, Njemačka, 15.03.1999. - 17.03.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
histopathology; PCP; kidney concretions; Planorbarius corneus; image analysis

Sažetak
The role of the excretory system of molluscs in bioaccumulation and depuration has still been insufficiently investigated. One of the peculiarities of molluscan kidneys is their ability to produce and excrete solid forms of waste material in the urine in form of concretions. It is thought that these concretions appear to be a normal formation of the excretory process of molluscs under pollutant, environmental or reproductive induced stress. Studies by Doyle et al and others point out that the kidney concretions may possibly serve as biological monitors of pollution. The purpose of this investigation was to determinate whether formation of concretions in the kidney of Planorbarius corneus is dose by dependent utilizing the automatic image analysis. Pentachlorophenol (PCP) was chosen as a reference compound because its chemistry, toxicology and fate in the environment is fairly well understood.Light microscopic histopathologic analysis of the PCP exposed P. corneus revealed increased formation of the kidney concretions, when compared to the kidneys of control animals. Multiple increase in the average number of kidney concretions is evident in all used concentrations. This kind of event has also been observed in the kidneys of Mercenaria mercenaria and Mytilus edulis under heavy metal stress and in land pulmonates rendered inactive by cold or drying. Light microscopical observations of Marigomez et al (12) on the excretory epithelium of Littorina littorea revealed that the amount of concretions increases at low Cd exposures (0.5 mg/L -10 days). The increase in the average surface of the concretions and in the average percentage of epithelial area filled with concretions, was clearly evident in all treated animals from the fourth day on. It is worthy noticing that on the second day, animals treated with the highest concentration of PCP showed significantly larger percentage of epithelial area filled with concretions than in the nontreated animals. Yevich also observed that quahogs (Mercenaria mercenaria, Arctica islandica, Mercenaria campehensis) collected from polluted areas had more and larger size concretions in the kidneys than those found in animals collected from non-polluted sources. The average surface of concretions in nontreated P. corneus ranged between 23 and 43.1 ľm2 (5.4 - 7.4 ľm in diameter respectively) similarly to the size of concretions (3-7 ľm in diameter) observed in Arion ater and excretion granules measuring 5-20 ľm in nephrocytes of Lymnaea stagnalis. The maximum observed concretion diameter for P. corneus in our experiment was 19.7 ľm. Kidneys of P. corneus might be able to serve as possible biological monitors but more research in this area is required.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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