Naslov
Lysosomal membrane damage in haemocytes of zebra mussel, Dreissena polymorpha exposed to sublethal concentrations of cadmium and copper.
(Lysosomal membrane damage in haemocytes of zebra mussel, Dreissena polymorpha exposed to sublethal concentrations of cadmium and copper)

Autori
Klobučar, Goran I.V. ; Maguire, Ivana ; Lucić, Andrea ; Kasap, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of SECOTOX 99, Munich, Njemačka, 65. / Kettrup, A. ; Schramm K.W. - München : GSF-Forschungszentrum fur Umwelt und Gesundhait, GmbH, 1999

Skup
Fifth European Conference on Ecotoxicology and Environmental Safety - SECOTOX 99

Mjesto i datum
München, Njemačka, 15.03.1999. - 17.03.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lysosome; haemocytes; Zebra mussel; Dreissena polymorpha; cadmium copper; NRR

Sažetak
Lysosomes concentrate a wide range of contaminants from within the environment, including lipophilic xenobiotics and metals, that may cause changes in the integrity of their membranes. Retention of the cationic probe neutral red within the lysosomal compartment was used as a measure of damage to the lysosomal membrane. The acid environment of lysosomes is maintained by the membrane Mg2+-ATPase dependent H+ ion proton pump. The neutral red retention assay (NRR) reflects the efflux of the lysosomal contents into the cytosol following damage to the membrane and, possibly, impairment of the H+ ion pump. Reductions in lysosomal membrane stability in digestive cells have already been recorded after experimental exposure of marine molluscs to copper and cadmium. The objective of this research was to investigate possible use of haemocyte lysosomes of freshwater bivalve Dreissena polymorpha as cellular marker of environmental pollution. The neutral red retention assay was adapted to assess the effects of cadmium and copper on haemocytes of D. polymorpha.However, our findings confirm that copper and cadmium have destabilizing effect on lysosomal membranes. Furthermore, haemocytes of another freshwater mollusc, snail Viviparus contectus, also showed significant decrease in NRR-time of lysosomes after 6 days of exposure to copper. NRR-time for zebra mussels from control group is shorter when compared to other species and remains to be clarified. It was found by Kraak et al that EC50 of Cu and Cd on the filtration rate of D. polymorpha were lower for Cu (41 ľg/L) than for Cd (388 ľg/L). Our research showed that, much lower cadmium concentrations cause damage to the mussel haemocytes. When we take into consideration the relevant environmental concentrations of investigated metals in oxic fresh waters, the results of this study point out copper as more toxic for zebra mussels than cadmium.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA