Naslov
QTL Analysis of Resistance to Mycosphaerella pinodes in Pea

Autori
Fondevilla, Sara ; Šatović, Zlatko ; Rubiales, Diego ; Moreno, Maria Teresa ; Torres, Ana Maria

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts. XIII EUCARPIA Biometrics in Plant Breeding Section Meeting. Agric. conspec. sci. 71(Suppl. 1) / Gunjača, Jerko ; van Eeuwijk, Fred A. ; Šatović, Zlatko - Zagreb : Agronomski fakultet Sveučilišta u Zagrebu, 2006

Skup
XIII EUCARPIA Biometrics in Plant Breeding Section Meeting

Mjesto i datum
Zagreb, Hrvatska, 30.08.-01.09

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pisum sativum L.; Mycosphaerella pinodes; disease resistance; genetic mapping; quantitative trait loci

Sažetak
Mycosphaerella pinodes causes necrotic spots on all aerial parts of the pea plant and is responsible for important yield and seed quality losses. The objective of this study was to gain a better knowledge of the genetic factors controlling pea resistance to M. pinodes. A population of 111 recombinant inbred lines (RILs), derived from the cross between P. sativum L. subsp. syriacum Berg (= P. humile Boiss and Nöe) line P-665 partially resistant to M. pinodes and the susceptible cultivar P. sativum L. cv. Messire was genotyped with morphological, isozyme, RAPD and STS markers. Reaction to M. pinodes was evaluated in both growth chamber and field conditions after inoculation with a single monosporic isolate from Córdoba, Spain. In growth chamber conditions disease rating was scored only on leaves according to a 0-5 scale (Ch) while in field conditions this disease rating scale was scored separately on leaves (L) and stems (St ; on a 0-5 scale). In addition disease severity was assessed in the field by a visual estimation of the percentage of plant area covered by symptoms (Sa). Flowering dates (DF) were also scored, as the number of days to 50% bloom. Of the 251 marker loci segregating in the RIL population, 246 could be mapped into nine linkage groups covering 1214 cM of the pea genome. Using composite interval mapping approach, five QTLs significantly associated with M. pinodes resistance were localized on linkage groups II, III, and V. Three QTLs (mp2, mp4, mp5) were detected on linkage group III. Some QTLs (mp1 and mp3) were detected only when disease was checked in growth chamber conditions (Ch), some in both chamber and field conditions (mp2 for Ch and Sa ; mp4 for Ch and St) while the QTL mp5 was identified only in field conditions (for L, St, and Sa). Four QTLs for flowering date were also detected. The major QTL detected in field conditions (mp5) explaining 31, 42, and 46% of the phenotypic variation of L, St, and Sa, respectively, co-localized with the major QTL for flowering date (df1).

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

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