Pregled bibliografske jedinice broj: 254737
Quercetagetin 3’ -methylether-7-O-ß-D-glucopyranoside - Containing Extracts from In vitro Grown Tissues of Centaurea rupestris L.
Quercetagetin 3’ -methylether-7-O-ß-D-glucopyranoside - Containing Extracts from In vitro Grown Tissues of Centaurea rupestris L. // Abstracts of the 11th IAPTC&B Congress "Biotechnology and Sustainable Agriculture 2006 and Beyond" / Scientific committe of the Congress (ur.).
Peking: Peking University and the Chinese Academy of Sciences, 2006. (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Quercetagetin 3’ -methylether-7-O-ß-D-glucopyranoside - Containing Extracts from In vitro Grown Tissues of Centaurea rupestris L.
Autori
Ćurković Perica, Mirna ; Krajačić, Mladen ; Rusak, Gordana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Abstracts of the 11th IAPTC&B Congress "Biotechnology and Sustainable Agriculture 2006 and Beyond"
/ Scientific committe of the Congress - Peking : Peking University and the Chinese Academy of Sciences, 2006
Skup
11th IAPTC&B Congress "Biotechnology and Sustainable Agriculture 2006 and Beyond"
Mjesto i datum
Peking, Kina, 13.08.2006. - 18.08.2006
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Centaurea rupestris L.; tissue culture; flavonoids; quercetagetin 3’ -metylether-7-O-ß-D-glucopyranoside; tomato bushy stunt virus
Sažetak
Centaurea rupestris L. is an endemic species which grows on the limited area of the Croatian coast with exclaves on Apennine and Balkan Peninsula. Flower extracts of Centaurea rupestris, as well as flavonoid quercetagetin 3’ -methylether- 7-O-ß- D-glucopyranoside isolated from the flowers, are known to have antibacterial, antifungal and antiphytoviral activity. In vitro clonal multiplication, rooting and acclimatization method was developed for this endemic species. The highest multiplication rate of 12 shoots per explant was achieved on Murashige and Skoog (MS) medium supplemented with 1 mM 6-benzylaminopurine and 2.9 mM gibberellic acid. Indole-3-butyric acid was the best root inducing auxin for C. rupestris. Rooting percentage achieved on half strength MS medium supplemented with 3 mM indole-3-butyric acid was 58%. Flavonoids were extracted by percolation in 45% and 90% ethanol and ether (1:1, w/v) from different plant tissues grown on MS medium supplemented with several combinations of plant growth regulators. The presence of quercetagetin 3’ -methylether-7-O-ß- D-glucopyranoside was confirmed by high performance liquid chromatography in all the tissues that developed shoots, but not in roots, transgenic roots or embriogenic callus. This result implies that synthesis of quercetagetin 3’ -methylether-7-O-ß-D-glucopyranoside is organ specific (flowers and shoots).
Izvorni jezik
Engleski
Znanstvena područja
Biologija
