Naslov
Effect of ß-Aminobutyric Acid and Indole-3-Butyric Acid on Phytoplasma-Infected Catharanthus roseus Shoots

Autori
Ćurković Perica, Mirna ; Šeruga Musić, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 11th IAPTC&B Congress &#8220 ; ; ; ; Biotechnology and Sustainable Agriculture 2006 and Beyond" / Scientific committe of the Congress - Peking : Peking University and the Chinese Academy of Sciences, 2006

Skup
11th IAPTC&B Congress &#8220 ; ; ; ; Biotechnology and Sustainable Agriculture 2006 and Beyond"

Mjesto i datum
Peking, Kina, 13.08.2006. - 18.08.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antiphytoplasmal activity; indole-3-butyric acid; periwinkle; tissue culture

Sažetak
Phytoplasmas are wall-less, non-helical prokaryotes that cause numerous economically important diseases infecting several hundred plant species worldwide. There are no effective ways to cure phytoplasma-associated diseases. Since phytoplasmas are uncultivable in vitro, phytoplasma reference strains are maintained in Catharanthus roseus shoots grown in vitro on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BA). In our experiments we examined the effect of ß-aminobutyric acid (BABA) and indole-3-butyric acid (IBA) on growth of periwinkle shoots infected with 3 different phytoplasma strains. In order to verify the phytoplasma presence in infected shoots, amplification of phytoplasma conserved 16S rRNA gene by nested PCR was used. BABA, a nonprotein amino acid that is known to exhibit postinfectional activity against nematodes, fungi, bacteria and tobacco mosaic virus, had positive effect on elongation of phytoplasma-infected shoots only in the first, but not in consequent subcultures. It had no effect on phytoplasma presence in infected tissues. Addition of IBA supported fresh weight increase and shoot elongation of all tested phytoplasma-infected shoots through all subcultures. However, there was no effect on the presence of phytoplasma strains EY-C (16SrV-A subgroup) and SA-I (16SrXII-A subgroup) in infected tissues. On the contrary, phytoplasma strain HYDB (16SrI-B subgroup) became undetectable in some of the tested shoots grown on media supplemented with different concentrations of IBA, showing that the phytoplasma titer in these samples dropped bellow the sensitivity limit of the nested PCR. This might imply that a change of exogenously supplemented hormone caused by transfer of the shoots from MS medium supplemented with BA to the one supplemented with IBA, could have caused a metabolic change unfavorable for HYDB phytoplasma growth.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA