Naslov
Polyketide gene-clusters in the Dictyostelium discoideum genome

Autori
Žučko, Jurica ; Long, Paul F. ; Cullum, John ; Hranueli, Daslav ; Kessin, Richard

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th International Symposium on the Genetics of Industrial Microorganisms : Book of Abstracts ; P018 / Paces, Vaclav - Prag : Czech Academy of Sciences, 2006, 54-54

Skup
International Symposium on the Genetics of Industrial Microorganisms (10 ; 2006)

Mjesto i datum
Prag, Češka Republika, 24.06.2006. - 28.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Dictyostelium discoideum; genome sequence; polyketide synthase gene-clusters; Type I genes

Sažetak
The social amoeba (cellular slime mould) Dictyostelium discoideum is an important model organism for studies in development, cell communication and recently evolution. Yet, one aspect of the life of these organisms has been ignored: like other soil micro-organisms they should produce a complex chemical repertoire. The genome sequence was recently completed showing around 12, 500 genes in a relatively small genome of 34 Mb. We have been analysing the potential polyketide synthase (PKS) clusters using bioinformatics methods. Similarity searches revealed 43 potential PKS genes and these were analysed further using an approach based on HMMER profiles. Twenty nine of the PKS genes occur in 12 potential gene-clusters (containing 2-5 genes each), which presumably correspond to the biosynthesis pathways of different natural products. Most of the genes are represented in EST libraries or microarray signals indicating that the pathways are active. RT-PCR studies suggest that the genes tested are transcribed and also that they have different patterns of regulation during the elaborate development of the organism. The genes show a similar domain structure to bacterial Type I PKS genes except that they contain a conserved additional 800 amino acid sequence of unknown function, which does not show any homology to any known protein. However, no loading domains could be identified, which suggests there might be a novel initiation mechanism. Twelve of the genes resemble Type I iterative genes, but no Type II iterative genes were found.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA