Naslov
Bcr-abl/abl ratio and FISH positivity - relation to c-kit expression and dual esterase activity in CML patients on Gleevec therapy

Autori
Radić Antolic, Margareta ; Sučić, Mirna ; Zadro, Renata ; Davidović-Mrsić, Sanja ; Marković-Glamočak, Mirjana ; Ries, Sunčica ; Gjadrov-Kuvezdić, Koraljka ; Vrbanus, Ljiljana ; Batinić, Drago ; Dubravčić, Klara ; Juričević, Mirjana ; Labar, Boris

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 11th Congress of the European Hematology Association ; u: Haematologica 91 (2006) (S1) / - , 2006, 481-481

Skup
Congress of the European Hematology Association (11 ; 2006)

Mjesto i datum
Amsterdam, Nizozemska, 15.06.2006. - 18.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bcr-abl/abl ratio; CD117 expression; Gleevec

Sažetak
Background: Chronic myelogenous leukemia (CML) is a myeloproliferative disease with t(9 ; 22) and/or BCR/ABL fusion gene. One of treatment options for CML patients is inhibition of BCR/ABL and c-kit (CD117) tyrosine kinase activity with imatinib mesylate (Gleevec). Aim of the study: To analyze CD117 expression and dual esterase activity in bone marrow hematopoietic cells (HCs) of CML patients on Gleevec therapy and to correlate the results with percentage of FISH Ph positive HCs and bcr-abl/abl ratio. Methods: 22 bone marrow specimens of 17 CML patients on Gleveec therapy (duration of therapy 4 – 32 months) were analyzed by FISH and quantitative RT-PCR, immunocytochemical APAAP CD117 expression and cytochemical dual esterase activity. Patients were divided in subgroups according to duration of therapy (less than 6, 6-12 and more than 12 months). Results: Patients with leukocyte differential count changes had high bcr-abl/abl ratio, FISH Ph and CD117 positive HCs. Medians of CD117 and FISH Ph positive HCs were highest in CML patients during the first 6 months of Gleveec treatment, but correlation of these two parameters was low (0.19). There was no statistical difference when medians of CD117 and dual esterase positive HCs were compared between subgroups. Correlation of FISH Ph positive HCs and bcr-abl/abl ratio was high (0.68) with constant decrease in percentage of FISH Ph positive HCs and bcr-abl/abl ratio during follow-up. Conclusions: According to low correlation obtained between CD117 expression and dual esterase activity with percentage of FISH Ph positive HCs and bcr-abl/abl ratio, FISH and quantitative RT-PCR are the methods of choice for monitoring the efficiency of Gleevec therapy.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA