Naslov
In vitro inhibition of macrophage spreading by antigens and lymphokines : Correlation with the footpad test and kinetics of inhibition = Inhibition de l'étalement des macrophages in vitro par les antigènes sensibilisants et les lymphokines : Cinétique d'inhibition et corrélation avec l'hypersensibilité cutanée de type retardé

Autori
Mažuran, Renata ; Silobrčić, Vlatko

Izvornik
Annales de l'Institut Pasteur. Immunology (0769-2625) 138 (1987), 2; 201-211

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
macrophage spreading inhibition ; lymphokine ; kinetics ; footpad test

Sažetak
As inhibition of spreading of mouse peritoneal macrophages is the basis for an in vitro test of cellular immunity, this test was used to investigate the correlation between in vitro and in vivo results and the kinetics of inhibition of spreading. BALB/C and NIH strain mice were immunized with human or bovine serum albumin or bovine gamma globulin. They displayed a positive footpad test when challenged with the specific antigen (21 days later). Peritoneal cells (PC) of mice with a strong footpad reaction were used for the spreading inhibition test. Intensity of spreading inhibition correlated well (r=-0.93) with that of footpad reaction. Spreading inhibition had a cyclic pattern. A similar pattern was observed after incubation of PC with performed lymphokines.- The cyclic pattern was probably caused by a repeated action of lymphokines on spread macrophages, resulting in reversible and re-inducible inhibition

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA