Naslov
A comparison of the inhibition of leucocyte migration and monocyte spreading as in vitro assays for tuberculin hypersensitivity in man

Autori
Silobrčić, Vlatko ; Sabioncello, Ante ; Mažuran, Renata ; Dekaris, Dragan ; Kadrnka-Lovrenčić, Magda

Izvornik
Clinical and experimental immunology (0009-9104) 20 (1975), 2; 239-247

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
leucocyte migration ; monocyte spreading ; tuberculin hypersensitivity

Sažetak
The ability of leucocyte migrationand monocyte spreading inhibition test to detect tuberculin hypersensitivity was compared in the same twelve Mantoux-negative and fifteen Mantoux-positive persons. Tuberculine hypersensitivity expressed in vitro as migration or spreading inhibition, induced by 100 micrograms of PPD/ml, was assessed after 2 and 24, or 4 and 20 hr of incubation. A significant difference was found between negative and positive persons by migration inhibition at the early interval and the spreading inhibition at both intervals. When the two tests were compared on the basis of individual results, monocyte spreading inhibition appeared more discriminating (fewer results in the group of positive persons overlapped with those found among negative persons). Results of the monocyte spreading inhibition test correlated well with cutaneous reaction at both incubation intervals, while with migration inhibition the correlation was not so well expressed at either interval. Furthermore, a given change in the sakin reactivity of tuberculin-positive persons was reflected better in spreading inhibition than in migration inhibition indices. We conclude that the method of monocyte spreading inhibition compares favouravbly with the method of leucocyte migration inhibition, and it seems to be a suitable in vitro test for detection of tuberculin hypersensitivity in man.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

Napomena
Rad je na hrvatskom jeziku prezentiran na skupu 8. Kongres Jugoslavenskog društva za fiziologiju, održanom u Opatiji, Jugoslavija ; objavljen u Knjizi sažetaka 1973.g., str. 230.

POVEZANOST RADA