Naslov
Utjecaj šećera i apscizinske kiseline na sazrijevanje somatskih embrija Pančićeve smreke (Picea omorika (Panč.) Purk.)
(Effect of sucrose and abscisic acid in somatic embryo maturation of omorika spruce (Picea omorika (Panč.) Purk.))

Autori
Tramišak-Milaković, Tamara ; Mihaljević, Snježana ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Šesti kongres biologa Hrvatske s međunarodnim sudjelovanjem / Huber, Đuro - Zagreb : Hrvatsko biološko društvo, 1997, 176-177

Skup
Šesti kongres biologa Hrvatske s međunarodnim sudjelovanjem

Mjesto i datum
Opatija, Hrvatska, 22.09.1997. - 26.09.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
sazrijevanje somatskih embrija; Pančićeva smreka; apscizinska kiselina; šećeri
(maturation somatic embryos; Picea omorika; abscisic acid; sugars)

Sažetak
Somatic embryo maturation is a critical step in somatic embryogenesis. Quality and viability of somatic embryos determine their germination and healthy plantlet production. Embryo maturation of conifers depends on nutritive medium composition, particularly on type and concentration of sucrose, and abscisic acid (ABA) concentration. Here, the role of different sugars and different concentrations of ABA on embryo maturation of two phenotypic different tissues of omorika spruce were examined. Embryogenic tissue in mature zygotic embryos was induced on modified LP medium supplemented with 1% sucrose, 10 uM 2,4-D and 5 uM BAP. During maintenance of cultures on 1/2xLP with 1% sucrose, 5 uM 2,4-D and 2.5 uM BAP two types of embryogenic tissue (B and W) were distinguished on their morphogenic and anatomic characteristics. On maturation medium (1/2xLP with 3% sucrose and 20 uM ABA) B tissue had significantly higher capacity of maturation than tissue W. Embryo maturation depended on sugar molarity that is on osmotic pressure of medium as well as on the sugar concentration expressed in percentage. Optimal embryo maturation in lines of B type tissue was achieved on 3% glucose (with higher osmotic pressure than 3% sucrose). Maturation in lines W was not improved on any tested media. Higher concentration of sugar (6%) inhibited embryo maturation in the both types of tissue. Combination of two sugars (sucrose + maltose, glucose + maltose) was more suitable than single ones. The highest number of mature somatic embryos were obtained on media with 40 uM ABA, while 60 uM ABA inhibited maturation.

Izvorni jezik
Hrvatski

Znanstvena područja
Biologija

POVEZANOST RADA