Naslov
Distinct glutathione-dependent enzyme activities and a verapamil-sensitive binding of xenobiotics in a fresh-water mussel Anodonta cygnea

Autori
Kurelec, Branko ; Pivčević, Branka

Izvornik
Biochemical and biophysical research communications (0006-291X) 164 (1989), 2; 934-940

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
GSH ; Glutathione ; GST ; Glutathione transferase ; CDNB ; 1-chloro-2 ; 4-dinitro-benzene ; AAF ; 2-acetylaminofluorene ; MDR ; Multi-drug resistance
(GSH ; glutathione ; GST ; glutathione transferase ; CDNB ; 1-chloro-2 ; 4-dinitro-benzene ; AAF ; 2-acetylaminofluorene ; MDR ; multi-drug resistance)

Sažetak
A fresh-water mussel Anodonta cygnea, an aquatic invertebrate resistant to pollution, possesses an inherent high potential to bind 2-acetylaminofluorene onto membrane vesicles. This binding is saturable and trypsin- and verapamil- sensitive. Simultaneously, this mussel reveals a relatively high inherent activity of glutathione-dependent enzyme activities with a distinct spectrum of substrate affinities. Both these activities are similar to the elements of the molecular mechanism involved in the acquired multi-drug resistance phenomenon described in tumour cell-lines. The recognition that in organisms exposed to polluted waters a multi-xenobiotic resistance mechanism may be involved is essential for understanding both the biological impact of pollution and the development of methods for rational risk assessment in regulatory policy.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA