Naslov
Influence of indole-3-butyric acid and ß-aminobutyric acid on phytoplasma-infected Catharanthus roseus shoots grown in vitro

Autori
Ćurković Perica, Mirna ; Šeruga Musić, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 16th Congress of the International Organization for Mycoplasmology (IOM) / Ayling, Roger D. ; Citti, Cristine ; Nicholas, Robin A. - Cambridge : IOM, 2006

Skup
16th Congress of the International Organization for Mycoplasmology (IOM)

Mjesto i datum
Cambridge, Ujedinjeno Kraljevstvo, 09.07.2006. - 14.07.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antiphytoplasmal activity; indole-3-butyric acid; periwinkle; tissue culture

Sažetak
Until today, there are no effective means to cure phytoplasma-associated diseases. Tetracyclines are known for long to have a bacteriostatic effect on mycoplasmas and phytoplasmas, but recently there are only several studies concerning effects of different chemical compounds on symptoms in phytoplasma-infected plants. Since phytoplasmas are uncultivable in vitro, reference strains are maintained in Catharanthus roseus shoots grown in vitro on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BA). The aim of our research was to examine influence of indole-3-butyric acid (IBA) and ß-aminobutyric acid (BABA) on growth of periwinkle shoots infected with different phytoplasma strains and possible postinfectional activity of these compounds against phytoplasmas. Following reference strains were used: HYDB (16SrI-B subgroup), EY-C (16SrV-A subgroup) and SA-I (16SrXII-A subgroup) in experiments with IBA ; and AY-A (16SrI-F) and KVI (16SrIII-B) in experiments with BABA. Infected C. roseus shoots were transferred from the medium supplemented with BA to the medium supplemented with different concentrations of IBA or BABA.Weight (g) and length (cm) of periwinkle shoots were measured at the beginning and at the end of each subculture. Amplification of phytoplasma conserved 16S rRNA gene by nested PCR was used to verify the phytoplasma presence in tested shoots. BABA, a nonprotein amino acid that is known to exhibit postinfectional activity against nematodes, fungi, bacteria and tobacco mosaic virus, induced growth of phytoplasma-infected shoots only in the first, but not in consequent subcultures. It had no influence on phytoplasma presence in infected tissues. Addition of IBA had positive effect on fresh weight increase and shoot elongation of all tested phytoplasma-infected shoots through all subcultures. However, it had no influence on the presence of phytoplasma strains EY-C and SA-I. On the contrary, phytoplasma strain HYDB became undetectable in some of the tested shoots grown on media supplemented with different concentrations of IBA, showing that the phytoplasma titer in these samples dropped bellow the sensitivity limit of the nested PCR. Shoot elongation and weight increase in periwinkle shoots were accompanied with healthy appearance and remission of symptoms. After one year of IBA treatment, periwinkle shoots were retransferred to the medium supplemented with BA. Some of the shoots showing remission of symptoms during the IBA treatment permanently escaped the infection and remained negative when tested for phytoplasma presence. This might imply that a change of exogenously supplemented hormone caused by transfer of the shoots from MS medium supplemented with BA to the one supplemented with IBA, could have caused a metabolic change unfavorable for HYDB phytoplasma growth.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA