Naslov
Mathematical Modeling of Size Exclusion Chromatography

Autori
Zelić, Bruno ; Nesek, Boris

Izvornik
Engineering in Life Sciences (1618-0240) 6 (2006), 2; 163-169

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
size exclusion chromatography; mathematical model of the process; finite difference method

Sažetak
A mathematical model of the size exclusion chromatography (SEC) process in chromatographic columns has been developed. It considers the following three mass transfer processes in the SEC column: axial dispersion in the bulk-fluid phase, interfacial film mass-transfer between the stationary and mobile phases, and diffusion of solutes within the macro pores of the packing particles. Differential equations of the process model were solved by the finite difference method. Characteristics of the column and the packing particles (bed void volume fraction, particle porosity, accessible particle porosity) were obtained experimentally, as well as retention times of different molecules with known molecular weights. Experiments were performed with two different columns containing two different packing materials, Superdex 75 HR 10/30 and BioSep SEC S2000, respectively. The model has been validated by comparing theoretical and experimental retention times for the different columns.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA