Naslov
Diabetic neuropathy: role of phosphorylation.

Autori
Belenkov, Alexander ; Hashiguchi, Mitsuko ; Križ, Jasna ; Hasihiguchi, Toshio ; Maysinger, Dušica ; Pađen, Ante

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Society for Neuroscience Abstracts, Volume 24 / Spciety for Neuroscience - Washington (MD) : Society for Neuroscience, 1998

Skup
28th Annual Meeting Society for Neuroscience

Mjesto i datum
Los Angeles (CA), Sjedinjene Američke Države, 07.11.1998. - 12.11.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Sažetak
Diabetic neuropathy (DN) is associated with decarse in conduaction velocity of peripheral nerves presumably as a result of metabolic dysfunction that affect Na channel and Na,K, ATPase activity. The mecahnism of these defects is not known but it may involve changes in phosphorylation of ion channles and/or neurofilaments (NF). To identify possible defcts inphosphryaltion pattern in peripheral nerves of diabtec animals. Electrophysiological and biochemical studies of peripheral nerves excised from streptozotocin-induced diabetic rats (STZ rats; 3 m post injection).OA (100nM), but not pVa (1-100 uM), had differential effect on compound action potentials (CAP) of sciatic nerves: it decreased amplitude ( 30%) recorded from normal animals but no change in CAP amplitude fromdiabetic preparation. Intra-axonal microelectrode recording from large myelinated axons showed decreased resting membrane potential and decreased inward rectification in STZ rats (similar to recent findings in human DN). Expression of NF-H is very high in DRG and low in the sciatic nerve (in control and STZ rats). In the presence of OA (50 nM, 30 min) and pVa (1-100 uM), there is a significantly stronger signal of NF-HP in DRG of diabetic rats whereas there is no significant enhancement of the signal in the sciatic nerves of diabetic rats. However, DN reveals high degree of NF-HP, both in the absence and with OA. These findings suggest a defect in regulation of phosphorylation of NF-H and Na* channels (K* channels ?) in the peripheral nerves of diabetic rats. This model system may be useful for further studies of DN mechanisms and for development of therapeutic interventions.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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