Naslov
Auxin-Amidohydrolase: a Regulator of Auxin Homeostasis in Plants

Autori
Tomašić, Ana ; Magnus, Volker ; Salopek-Sondi, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EMBO/HHMI Central European Scientists Meeting 2006. Conference Proceedings / - , 2006, 82-82

Skup
EMBO/HHMI Central European Scientists Meeting

Mjesto i datum
Cavtat, Hrvatska, 15.06.2006. - 17.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
auksin-amidohidrolaza; pšenica; indol-maslačna kiselina
(auxin-amidohydrolase; wheat; indol-butiric acid)

Sažetak
The wheat auxin-amidohydrolase, TaIAR3, was investigated. TaIAR3 cDNA was isolated from winter wheat (Triticum aestivum L. cv. Caledonia) and was obtained as a gift from Dr. James Campanella, Montclair State University, NJ, USA. The wheat auxin-amidohydrolase gene was cloned into several expression vectors and protein expression was obtained in E. coli. To determine enzyme activity and substrate specificity, the bacterial cell lysate was processed for biochemical reaction with auxin-amino acid conjugates as putative substrates, and the auxin formed was monitored by HPLC. HPLC separation showed specific cleavage of the, biologically inactive, alanine conjugate of indolebutyric acid (IBA-Ala) to form the, physiological active, free IBA. This fact suggests that wheat auxin-amidohydrolase is involved in auxin homeostasis in plants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA