Naslov
Functional Long-Term Expression of delta12 Desaturase in Yeast is Associated with Adaptation to Oxidative Stress

Autori
Čipak, Ana ; Hasslacher, Meinhard ; Jaganjac, Morana ; Collinson, Emma J. ; Tehlivets, Oksana ; Borović, Suzana ; Waeg, Georg ; Dawes, Ian W. ; Žarković, Neven ; Kohlwein, Sepp D.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the HNE-Club Meeting "Role of 4-Hydroxynonenal and Related Lipid Peroxidation Products in Cell Pathophysiology" / - Genova, 2006, 50-50

Skup
HNE-Club Meeting "Role of 4-Hydroxynonenal and Related Lipid Peroxidation Products in Cell Pathophysiology"

Mjesto i datum
Genova, Italija, 16.06.2006. - 18.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HNE; oxidative stress; delta 12 desaturase

Sažetak
PUFA peroxidation plays an important role in various diseases such as atherosclerosis, (neuro)degenerative diseases, fibroproliferative diseases and cancer. Although these diseases have different pathology, they all include cellular alterations of PUFA metabolism and peroxidation, which are not well understood. Therefore, it is of importance to create simple experimental systems for studying lipid peroxidation on the molecular and cellular levels. For this purpose, we have developed a novel system based on yeast that is expressing a heterologus delta12 desaturase gene, which allows induced PUFA synthesis in a cell that normally does not produce these fatty acids. Although functional expression of the desaturase did not affect cellular morphology or growth in the absence of pro-oxidants, low levels of HNE-protein adducts were detected in a strain expressing the desaturase gene ; HNE-protein conjugate production was greatly enhanced in the presence of H_2O_2, with a concomitant decrease in cell viability, under conditions of desaturase expression. Furthermore, we observed difference in sensitivity to oxidative stress depending on the duration of the desaturase expression. Initially the mutants were more sensitive to oxidative stress than the wild type cells, but their increased sensitivity was reversible. This change in oxidative stress sensitivity was reflected in a change of the cellular oxidative and antioxidative parameters. Evidence suggests that the yeast expressing the heterologous desaturase gene adjusted to low level of endogenous lipid peroxidation and consequentially, became more resistant to exogenous oxidative stress.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA