Naslov
Exonuclease requirements for recombination of lambda phage in recD mutants of Escherichia coli

Autori
Đermić, Damir ; Zahradka, Davor ; Petranović, Mirjana

Izvornik
Genetics (0016-6731) 173 (2006), 4; 2399-2402

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
exonucleases; ExoI; ExoVII; ExoX; RecJ; SbcCD; RecBC enzyme; lambda lytic crosses

Sažetak
Recombination of lambda red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5'-3' exonuclease activity of RecJ and exonuclease VII is essential for lambda recombination, whereas exonucleases of 3'5' polarity are dispensable. The implications of the presented data on current models for recombination initiation in E. coli are discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA