Naslov
Pomaceous fruit tree phytoplasmas and their potential vectors in Croatia

Autori
Križanac, Ivana ; Mikec, Ivan ; Budinščak, Željko ; Šeruga Musić, Martina ; Krajačić, Mladen ; Škorić, Dijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the 20th International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops and 21st International Symposium on Small Fruit Virus Diseases / Scientific Comittee of the Symposium - Antalya, 2006

Skup
20th International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops and 21st International Symposium on Small Fruit Virus Diseases

Mjesto i datum
Antalya, Turska, 22.05.2006. - 27.05.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pear decline; psyllids; 16S rDNA; PCR; RFLP

Sažetak
Surveys of pomaceous fruit trees were carried out in the years 2003 and 2004 in order to determine the current status of 'Candidatus Phytoplasma pyri' (pear decline, PD) and 'Ca. P. mali' (apple proliferation, AP) in Croatia. PD and AP were in the focus of our interest because they are on the Croatian A2 quarantine list of pests and diseases. Eleven orchards in continental and coastal fruit growing regions were visually inspected for the symptom presence during summer and autumn. Leaf and twig samples were collected from symptomatic pear and apple trees. In 2004, phytoplasma vectors Cacopsylla pyri, C. pyrisuga and C. pyricola were collected from 5 orchards in continental and coastal Croatia by using entomological nets and yellow sticky traps. Nucleic acids extracted from insects and pome fruit trees were PCR-tested using phytoplasma universal primers R16F1/R0 for direct and R16F2n/R2 for the first nested PCR. Amplicons digested with Tru9I (MseI) showed profiles consistent with the presence of phytoplasmas belonging to one of the ribosomal groups (16SrX, 16SrXII, 16SrI), except in two cases where mixed infections were detected. For samples showing profiles typical of 16SrX group, additional digestion with RsaI and SspI was done to determine the ribosomal subgroup lately designated as 'Ca. P. pyri', 'Ca. P. mali' and 'Ca. P. prunorum'. Second nested PCR assays by using group specific primer pairs R16(X)F1/R1 and R16(I)F1/R1 were also performed. So far, the presence of following 'Candidatus Phytoplasma' species has been confirmed: -'Ca. P. pyri' in pear, apple, Cacopsylla pyri and C. pyrisuga, -stolbur (16SrXII-A) in pear, apple, Cacopsylla pyri, C. pyrisuga and C. pyricola, as well as -'Ca. P. asteris' (16SrI-B) in pear and Cacopsylla pyri. In this research, we have found no molecular evidence for the presence of 'Ca. P. mali' in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

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