Naslov
Quantitative analysis of constitutive heterochromatin in couples with fetal wastage

Autori
Buretić-Tomljanović, Alena ; Radojčić Badovinac, Anđelka ; Vlastelić, Ivica ; Randić, Ljiljana

Izvornik
American Journal of Reproductive Immunology (0271-7352) 38 (1997), 3; 201-204

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
abortion; spontaneous; heterochromatin

Sažetak
Heteromorphism of constitutive heterochromatin is stable evolutionaty feature that is thought to cause no phenotypic alteration. Neverheless, the role of constitutive heterochromatin is still unknown. The instability of constitutive heterochromatin was generally restricted to T-lymphocytes and was associated with variable immunnodeficiency. The heterochromatin regions of chromosome 1, 9, 16, and y have been postulated to play a role in immune response and during early embryo development. To investigate a possible influence of constitutive heterochromatin in human reproductive ability, quantitative analysis of constitutive heterochromatin in human chromosomes 1, 9, 16, and Y was done. Thirty couples were divided into two groups, owing to the clinical heterogeneity of their reproductive disorders. The first group included couples with two or more spontaneous abortion as the only pregnancy outcomes, and the second group included couples with stillborn child with or without malformation. In the controle group were couples with one or more healthy children without a history of fetal westage. All the persons in this study had normal karyotypes. The amount of constitutive heterochromatin was expressed by relative value using the simple transformation q/(p+q). This value, obtained on GTG-banded metaphase chromosomes, represented an indirect measure of heterochromatin content. The Y/F index was used to express the relative amount of heterochromatin in chromosome Y. There was significant increase in heterochromatin content of the chromosome 16 homologue pair in males and females with stillborn or a stillborn malformed child (P<0.01) and a increase in total heterochromatine cell content comared to controls (P=0.005). The same couples had significantly increased mean maximal heterochromatin content in the potential zygotes (P<0.02). The couples who experienced spontaneous abortions only had a minimal total heterochromatin content in the poteintial zygotes (P<0.05). The Y/F index was significantly ower in the males in both groups compared to controls (P1<0.02 ; P2<0.02). The quantitative analysis of constitutive heterochromatin could be valuable in predicting pregnancy outcome.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA