Naslov
T cell CD3-zeta-eta heterodimer expression and coupling to phosphoinositide hydrolysis

Autori
Merćep, Mladen ; Bonifacino, Juan S. ; Garcia-Morales, Pilar ; Samelson, Lawrence E. ; Klausner, Richar D. ; Ashwell, Jonathan D.

Izvornik
Science (0036-8075) 242 (1988), 4878; 571-574

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
activation ; phosphoinositide ; hydrolysis ; T cells ; T cell receptor

Sažetak
The T cell antigen receptor consists of an antigen-binding heterodimer that is noncovalently associated with at least five CD3 subunits (gamma, delta, epsilon, zeta, and eta). The CD3-zeta chains are either disulfide-linked homodimers (CD3-zeta 2) or disulfide-linked heterodimers with eta (CD3-zeta eta). Variants of a murine antigen-specific T cell hybridoma that express normal amounts of CD3-zeta 2 but decreased amounts of CD3-zeta eta were isolated. When activated, the parental cell line increased both phosphatidylinositol hydrolysis and serine-specific protein kinase activity to a much greater extent than the variants. In contrast, the activation of a tyrosine-specific kinase after stimulation with a cross-linking antibody to CD3 was similar among these cells. There was a positive linear relation between the expression of CD3-zeta eta and phosphoinositide hydrolysis stimulated by the TCR, suggesting a differential coupling of the T cell alpha beta heterodimer to signal transduction mechanisms due to alpha beta association with either CD3-zeta 2 or CD3-zeta eta

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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