Naslov
Role of the zeta chain in the expression of the T cell antigen receptor: genetic reconstitution studies

Autori
Weissman, Allan M ; Frank, Stuart J ; Orloff, David G ; Merćep, Mladen ; Ashwell, Jonathan D ; Klausner, Richard D

Izvornik
EMBO journal (0261-4189) 8 (1989); 3651-3656

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
assembly ; degradation ; signal transduction ; transcription ; T cell receptor

Sažetak
The zeta (O) chain plays a central role in T cell antigen receptor assembly and signal transduction. From previous work in murine T cell hybridomas we have inferred that the v subunit is limiting in receptor assembly. Partial receptors made in excess of r are assembled in the endoplasmic reticulum, transported through the Golgi, but then rapidly and efficiently degraded in lysosomes. r would therefore seem to play a unique role in targeting receptors from the Golgi to the cell surface. To determine directly whether s limits receptor assembly we have reconstituted a ¢ ; ; - deficient T cell line by transfection of the murine r cDNA. Transfection results in restoration of expression of surface T cell receptor. In addition, increasing r expression results in a commensurate increase in the survival of previously excess subunits. This is reflected in an increased surface expression of complete receptors. Finally, transfection of the r cDNA fails to produce detectable P-i7 heterodimers. The implications of these findings with regard to receptor assembly, and the relationship between r and v7, are discussed. Key words: assembly / degradation / signal transduction/

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA