Naslov
OPTIMIZATION OF THE GROWTH MEDIUM FOR EXTRACELLULAR LIPASE PRODUCTION BY MOLDS Aspergillus nlger

Autori
Radulić, Marina ; Šulić, Sanda ; Bešlić, Jasminka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
6.European Students' Conference of the Charite

Mjesto i datum
Berlin, Njemačka, 19.10.1995. - 21.10.1995

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aspergillus; mold; lipase

Sažetak
Lipase (EC 3.1.1.3) hydrolyzes trlacyiglicerol to fatty acid and glycerol and has been found in various microorganisms. The aim of this work was to study the optimal growth medium and the maximum activity of extracellular lipase produced by Aspergillus nlger (AQ5). Aspergillus nlger showed maximum growth and lipase production at 30°C in 3 days at pH 5, 5. The medium supplemented with glucose showed maximum production of lipase followed by sucrose, mannitd, maltose and lactose. Amongst the nitrogen sources tested, lipase yield was maximum with pepton at 1% level. Ammonium chloride Increased the yield of lipase, whereas calcium chloride stimulated the enzyme activity. Lipase activity was determined (the substrate were olive oil) by titration with 0.02 M NaOH with indicator phenolphtaleln. The highest enzyme activity (18 U/mL) was on medium supplemented with: peptone 1%. glucose 2%, sunflower oil 1%.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA