Naslov
Effect of ß-aminobutyric acid on phytoplasma-infected Catharanthus roseus shoots

Autori
Ćurković Perica, Mirna ; Šeruga Musić, Martina

Izvornik
Journal of Plant Diseases and Protection (0340-8159) 112 (2005), 5; 544-549

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antiphytoplasmal activity; ß-aminobutyric acid; periwinkle; tissue culture

Sažetak
SS-aminobutyric acid (BABA) is a simple, nonprotein amino acid that exhibits postinfectional activity against nematodes, fungi, bacteria and TMV, but it’ s activity against phytoplasmas has never been examined before. The aim of this research was to examine possible postinfectional activity of BABA against phytoplasma reference strains AY-A and KVI in Catharanthus roseus shoots grown in vitro. Shoots were grown on MS medium supplemented with different concentrations of BABA (10 mM, 100 mM, 1 mM, 10 mM, 100mM), through 5 subcultures. The length of the shoots infected with phytoplasma strain AY-A was measured (in mm) at the beginning and at the end of the first three subcultures. Only in the first subculture BABA supported shoot elongation. The presence of phytoplasmas was confirmed by nested PCR in both AY-A and KVI-infected shoots, after the first and the fifth subculture, showing that in these experimental conditions BABA had no curative effect against phytoplasmas.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA