Pregled bibliografske jedinice broj: 236833
Extracellular metalloendopeptidase of Streptomyces rimosus
Extracellular metalloendopeptidase of Streptomyces rimosus // Archives of microbiology, 185 (2006), 3; 183-191 doi:10.1007/s00203-006-0084-x (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 236833 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Extracellular metalloendopeptidase of Streptomyces rimosus
Autori
Vitale, Ljubinka ; Vukelić, Bojana ; Križaj, Igor
Izvornik
Archives of microbiology (0302-8933) 185
(2006), 3;
183-191
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Metalloendopeptidase ; Specificity ; Amino acid esquence ; Streptomyces rimosus
(Metalloendopeptidase ; Specificity ; Amino acid sequence ; Streptomyces rimosus)
Sažetak
Metalloendopeptidase was isolated from Streptomyces rimosus culture filtrates in a homogeneous form. It was determined to be a 15 kDa basic protein, most active around pH 7.5, and susceptible to inhibition by chelating agents, N-bromosuccinimide, thiorphan, and 10^-^4M zinc. The enzyme was highly specific for phenylalanine at the N-side of endopeptide bonds. Determination of amino acid sequence of the enzyme^, s NH_2-part allowed the recognition of its structure homology with several Streptomyces species. The data contribute to the definition of M7 family of metalloendopeptidases in streptomycetes.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
