Naslov
Aflatoxigenicity of Rhizopus nigricans strains isolated from drug plants

Autori
Pepeljnjak, Stjepan ; Cvetnić, Zdenka

Izvornik
Acta pharmaceutica (1330-0075) 48 (1998), 2; 1139-144

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mycological contamination; drug plants; Rhizopus nigricans; aflatoxin B1; aflatoxin B2
(Mycological contamination; drug plants; Rhizopus nigricans; Aflatoxin B1; Aflatoxin B2)

Sažetak
Mycological analyses of 64 samples of drug plants stored and collected from drugstores showed 50% of samples to be contaminated with Rhizopus nigricans. Aflatoxigenicity of Rhizopus nigricans strains was assessed by specific fluorescence test in aflatoxin-producing ability medium (APA) (detection in UV at 365 nm) and by extraction after biosynthesis on yeast extract sucrose (YES) broth. Aflatoxins were produced by 40% of strains. All of the aflatoxins-producing strains in YES showed intensive blue fluorescence in the APA medium. The strains of Rhizopus nigricans were producers of two aflatoxins :AFLB1 and AFLB2. The concentrations of aflatoxin B1 and aflatoxinB2 ranged from 1.22 to 1.33 and from 0.1 to 0.2 mg/L culture filtrate, respectively. The results warn of the risk of improper storing and using drug plants with no previous evaluation of their quality in terms of mycotoxicological parameters.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA