Naslov
Effects of proteinase inhibitors on western corn rootworm life parameters

Autori
Bažok, Renata ; Igrc Barčić, Jasminka ; Edwards, C. Richard

Izvornik
Journal of Applied Entomology (0931-2048) 129 (2005), 4; 185-190

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
proteinase inhibitors; E-64; Kunitz trypsin inhibitor; PMSF; western corn rootworm; fecundity; life span

Sažetak
Plants have developed defensive mechanisms to minimize predation by insect pests. Proteinase inhibitors are one example of plant compounds synthesized as a mechanism for defence. The objective of this study was to determine the impact of the E-64, PMSF, and Kunitz trypsin inhibitors on the preovipositional and ovipositional periods, the mean number of eggs laid per female, and the longevity of western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, adults. This study provides information on the effectiveness of proteinase inhibitors as a host plant resistance tool for managing WCR beetles. The study was conducted in 1997, 1998, and 2000. In 1997, E-64 was added to an artificial diet at the concentrations of 0.05%, 0.025%, and 0.0125% (w/w), corresponding to 500, 250, and 125 ppm, respectively. In 1998, PMSF was added to the artificial diet at the same concentrations. In 2000, Kunitz trypsin inhibitor was added to the artificial diet at concentrations of 0.2%, 0.1%, and 0.05% (w/w), corresponding to 2000, 1000, and 500 ppm, respectively. The mean fecundity of beetles fed the untreated diet was between 67 and 111 eggs per female. The fecundity of beetles fed E-64 and PMSF at different concentrations, ranged between 162 and 246 eggs per female for E-64 and 61 and 80.5 eggs per female for PMSF. The fecundity of the beetles fed Kunitz trypsin inhibitor was between 155 and 225 eggs per female. When beetles fed on the diet which consisted of the lowest dosage of Kunitz trypsin inhibitor (500 ppm), fecundity was higher than on untreated control. The proteinase inhibitors investigated did not show negative impact on WCR adults. Beetle fecundity, the length of the preovipositional and ovipositional periods and the longevity of the beetles fed with proteinase inhibitors were not lower than the beetles fed only the artificial diet. This study does not support the use of investigated proteinase inhibitors at applied concentrations as effective host plant resistance tools for managing WCR beetles.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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