Naslov
Solid phase extraction and HPLC determination of veterinary pharmaceuticals in wastewater

Autori
Babić, Sandra ; Ašperger, Danijela ; Mutavdžić, Dragana ; Horvat, Alka J.M. ; Kaštelan-Macan, Marija

Izvornik
Talanta (0039-9140) 70 (2006), 4; 732-738

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
pharmaceuticals ; wastewater ; high-performance liquid chromatography ; diode array detection ; soild-phase extraction

Sažetak
This work focuses on the application of SPE-HPLC analysis of important veterinary pharmaceuticals from different classes in highly complex wastewater matrix. The pharmaceutical investigated included three sulfonamides (sulfamethazine, sulfadiazine and sulfaguanidine), a sulfonamide synergist (trimethoprim), a tetracycline (oxytetracycline), a fluoroquinolone (enrofloxacine) and a  -lactame (penicilline G / procaine). The method involves pre-concentration and clean-up by soild phase extraction (SPE) using Oasis HLB extraction catridges. Final analysis of the selected pharamceutical compounds was carried out by high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD). Recoveries were ranged from 68.3 to 97.9 % with relative standard deviation below 8.4 %. Only for sulfaguanidine low recovery was obtained. Limits of quantification were in the range 1.5-100  g/L depending on pharmaceutical. The described method was applied to the determination of pharmaceuticals in wastewater samples from pharmaceutical industry.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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