Naslov
Molecular epidemiology of extended-spectrum beta-lactamases from Klebsiella pneumoniae strains, isolated in Zagreb, Croatia

Autori
Bedenić, Branka ; Randegger, Corrinne ; Stobberingh, Ellen ; Hachler, Herbert

Izvornik
European Journal of Clinical Microbiology and Infectious Diseases (0934-9723) 20 (2001); 505-508

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
extended-spectrum beta-lactamases; Klebsiella pneumoniae; molecular epidemiology

Sažetak
In order to assess the molecular epidemiology of 40 previously identified extended-spectrum beta-lactamases (ESBL)- producing clinical isolates of Klebsiella pneumoniae, gene sequencing was performed. While the previous examination of these isolates revealed one TEM producer, the sequencing procedure performed in this study identified 13 additional TEM producers, and all of the sequenced genes reflected production of non ESBL TEM-1. All 38 suspected SHV producers were confirmed to be carriers of blaSHV-ESBL genes using PCR/Nhe test and sequencing. Among the, types SHV-2, SHV-5 and SHV-12 were found in 20, 10 and / isolates, respectively, and SHV-2a was identified in 1 isolate. SHV-5 and SHV-12 conferred higher resistance to ceftazidime and cefepime, while SHV-2 and SHV-2a raised the minimum inhibitor concentrations of cefotaxime and cefpirome. Fourth generation cephalosporins were found to be more active against the isolates than third-generation cephalosporins.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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