Naslov
Sporulation in diploid transformants of Saccharomyces cerevisiae obtained by illegitimate integration of single-stranded DNA

Autori
Gjuračić, Krešimir ; Zgaga, Zoran

Izvornik
Food technology and biotechnology (1330-9862) 36 (1998), 2; 89-93

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Saccharomyces cerevisiae; illegitimate recombination; sporulation

Sažetak
DNA sequence analysis and chromosomal polymorphism of natural and industrial yeast strains reveal important role of illegitimate recombination in the evolution of the yeast genome. The resulting genetic changes can be brought to the homozygous state by sporulation and mating between haploid progeny. In this work we compared sporulation of diploid transformants obtained by illegitimate integration of homologous single-stranded (ss) DNA and transformants obtained with double-stranded (ds) plasmid that replicates autonomously in the yeast cell. The transformants that contained exogenous DNA integrated by illegitimate recombination had lower efficiency of sporulation, but the spore viability was decreased from 80% to 65% only among preselected fraction of transformants. Some of the surviving haploids contained transforming DNA integrated in their genome. Possible mechanisms that led to the decrease in sporulation efficiency and spore viability in transformants obtained by illegitimate integration of foreign DNA into yeast genome are discussed.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA