Naslov
Homologous Recombination and DNA Repair in recBC sbcBC Mutants of Escherichia coli: Effects of a recG Mutation

Autori
Zahradka, Davor ; Buljubašić, Maja ; Zahradka, Ksenija ; Petranović, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 1st Central European Forum for Microbiology (CEFORM) ; u: Acta Microbiologica et Immunologica Hungarica. Supplement 52 (2005) (S) / Marialigeti, K. ; Sipos, R. - Budimpešta : Akadémiai Kiadó, 2005, 174-175

Skup
Central European Forum for Microbiology (1 ; 2005)

Mjesto i datum
Keszthely, Mađarska, 26.10.2005. - 28.10.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
homologous recombination; DNA repair; recBC sbcBC mutants; recG mutation

Sažetak
RecG protein is a DNA helicase that promotes DNA strand exchange and heteroduplex extension during homologous recombination in Escherichia coli. Cells mutated in the recG gene show moderate recombination deficiency and increased UV-sensitivity compared to the wild type cells. We have studied the effects of a recG mutation on recombinational DNA repair after exposure to UV-irradiation, on conjugal recombination, and on cell morphology in two types of recBC sbcBC mutants of E. coli. In one mutant, the sbcB gene was inactivated by point mutation (sbcB15), while in other it was completely deleted (delsbcB). We found that introduction of the recG mutation in a recBC sbcB15 sbcC background strongly reduced the efficiency of recombination and recombinational DNA repair, and caused severe defects in chromosome segregation and cell division. On the contrary, in recBC delsbcB sbcC background, the recG mutation had relatively mild effect on both recombination processes and cell morphology. We inferred that the sbcB15 mutation modifies recombinational process in a way that the process becomes more dependent on the RecG protein. It is possible that the mutant SbcB15 protein binds to recombinogenic 3' DNA ends and interferes with the processing of recombination intermediates in the absence of RecG protein.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA