Pregled bibliografske jedinice broj: 228591
Kinetic modeling of amino acid oxidation catalyzed by a new D-amino acid oxidase from Arthrobacter protophormiae
Kinetic modeling of amino acid oxidation catalyzed by a new D-amino acid oxidase from Arthrobacter protophormiae // Engineering in life sciences, 5 (2005), 6; 550-555 doi:10.1002/elsc.200520105 (međunarodna recenzija, članak, znanstveni)
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Naslov
Kinetic modeling of amino acid oxidation catalyzed by a new D-amino acid oxidase from Arthrobacter protophormiae
Autori
Findrik, Zvjezdana ; Vasić-Rački, Đurđa ; Geueke, Birgit ; Kuzu, Mutlu ; Hummel, Werner
Izvornik
Engineering in life sciences (1618-0240) 5
(2005), 6;
550-555
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
D-amino acid oxidase ; enzyme kinetics ; modelling
Sažetak
D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was kinetically characterized using its natural substrate D- methionine and aromatic amino acid 3, 4-dihydroxyphenyl-D-alanine (D- DOPA). Kinetic measurement revealed high specificity of this enzyme to D-methionine (Km = 0.24 mM) and lower specificity on D-DOPA as a substrate (Km = 9.33 mM). The enzyme showed no activity to L-methionine and L-DOPA. Batch and repetitive batch experiments were performed with both substrates in the presence and absence of catalase for hydrogen peroxide decomposition.
Izvorni jezik
Engleski
Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
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