Naslov
Enumeration of haemagglutinin-specific CD8+ T cells after influenza vaccination using MHC class I peptide tetramers

Autori
Kosor, Ela ; Gagro, Alenka ; Draženović, Vladimir ; Kuzman, Ilija ; Jeren, Tatjana ; Čečuk-Jeličić, Esma ; Kerhin-Brkljačić, Vesna ; Gjenero-Margan, Ira ; Kaić, Bernard ; Rakušić, Snježana ; Sabioncello, Ante ; Markotić, Alemka ; Rabatić, Sabina ; Mlinarić-Galinović, Gordana ; Dekaris, Dragan

Izvornik
Scandinavian journal of immunology (0300-9475) 67 (2007), 1; 86-94

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
influenza; vaccine; MHC class I tetramers; CD8+ T cells

Sažetak
With emergence of MHC class I tetramers loaded with CD8+ T-cell viral epitopes, it is possible to study virus-specific CD8 cells in humans during infection and after vaccination. MHC class I tetramers was used to detect the frequency of haemagglutinin (HA)-specific T cells in 26 healthy influenza-vaccinated humans. Peripheral blood was collected before, and 7, 14 and 28 days after vaccination. Four-colour flow cytometry was used for monitoring of vaccine induced T-cell response. In 15 donors, two- to fivefold increase in frequency of HA-specific T cells was observed 7 days after vaccination. In addition, in 12 of these donors, this increase was accompanied with fourfold increase of H1N1 antibody titre. The increase in frequency of HA-specific CD8+/IFN-gamma+ cells was low and peaked 28 days after vaccination in three of the six donors tested. Frequencies of HA-specific CD8+ T cells and antibody titre returned to prevaccination values 1 year after vaccination. Subunit influenza vaccines have the ability to induce HA-specific CD8+ cells. As the immune response to this vaccine decreased significantly after 1 year, our results confirm the importance of annual immunization for adequate protection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

Napomena
Dio istraživanja opisanih u radu financiran je i sredstvima projekta TP-01/0021-05 (voditelj Alenka Gagro).

POVEZANOST RADA