Naslov
Sendai virus propagated in different cell substrates may induce different immunoreactions in peripheral blood leukocytes

Autori
Markotić, Alemka ; Cvetko, Lidija ; Cebalo, Karin ; Cebalo, Ljiljana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Godišnji sastanak Hrvatskog imunološkog društva

Mjesto i datum
Božava, Hrvatska, 29.09.2005. - 02.10.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Sendai virus; fibroblasts; embryonated chicken eggs; immunoreactions; cytokines/chemokines

Sažetak
Sendai virus (SeV), a negative-strand RNA virus that belongs to the Paramyxoviridae family, is usually propagated in embryonated chicken eggs. Previously, we showed that Sendai virus could be propagated in human lung fibroblasts (MRC-5 cells) in one passage and could induce production of several different cytokines/chemokines. It could be a strong inductor of alpha interferon (IFN- ) in peripheral blood leukocytes (PBL), but is also capable of suppressing the antiviral action of IFN- / . Interferons IFN- / and IFN- act through independent cell-surface receptors, inducing gene expression through tyrosine phosphorylation of cytoplasmic transcription factors. The aim of our study was to investigate possible differences in immunoreactions induced in PBL infected with SeV propagated in MRC-5 cells (SeV-F) in comparison to SeV propagated in embryonated chicken eggs (SeV-E). For that purpose we infected PBL with 200 HAI of SeV-E or SeV-F, and incubated 18 h, together with non-infected PBL as controls. Total RNA was isolated from PBL and RT-PCR was used for the detection of gene expression for different IFN types and subtypes, as well as Janus tyrosine kinase (JAK)-STAT gene family. Cell supernatants were collected and tested using protein array testing (Human Antibody Array III and 3.1, RayBiotech, Inc., Norcross, GA, USA) including 42 different inflammatory proteins. Additionally, the levels of IFN- were detected using standard cytopathic effect inhibition bioassay and commercial ELISA test. SeV-F induced higher levels (~30%) of IFN- than SeV-E. SeV-E induced six-fold higher gene expression of IFN- 6 than SeV-F, which induced two-fold higher gene expression for IFN- . Both viruses decreased expression of JAK2, while only SeV-F decreased expression of Tyk2. Both viruses induced production of IL-6 and MIP-1 , while SeV-F induced IL-16 also. Our results showed that SeV propagated in different cell substrates may induce different immunoreactions in PBL.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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