Naslov
Studying the extent of agreement between the photoacoustic and optothermal methods for assessing the amount of lycopene in foods derived from processed tomatoes

Autori
Bicanic, Dane ; van der Veeken, Pascal ; van Rooij, Hans ; Dóka, Otto ; Luterotti, Svjetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd central European Meeting - 5th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, Opatija, 17-20. listopad 2004. Book of Abstracts / Karlović, Damir - Zagreb : Hrvatsko društvo za prehrambenu tenologiju, biotehnologiju i nutricionizam, 2005, 24-24

ISBN
953-99725-0-7

Skup
2nd Central European Meeting - 5th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, Opatija, 17-20. listopad 2004.

Mjesto i datum
Opatija, Hrvatska, 17.10.2004. - 20.10.2004

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
lycopene; detection; processed tomato; photothermal methods

Sažetak
The consumption of lycopene, major substance in tomato was frequently demonstrated to have beneficial health effects. Methods most commonly used to determine lycopene include various forms of chromatography and spectrophotometry. The main problem encountered in the analysis of lycopene is the need for a time consuming preparation of the sample as well high as considerable instability of this compound (in terms of exposure to heat and light, interaction with chemicals etc.). Recently, two novel laser based photothermal (PT) techniques, i.e. the optothermal window (OW) and photoacoustic spectroscopy (PAS) for determination of lycopene have been proposed. Both approaches enable direct (no sample preparation whatsoever) assessment of lycopene but the availability of a previously obtained calibration curve is a necessity. In this study OW and PAS were used to rapidly assess lycopene in a variety of commercial products (tomato juice, ketchup, pastas and purees) derived from processed tomatoes. Absolute concentrations of lycopene in these products were determined by HPLC and spectrophotometry. The OW and PAS measurements were performed with the argon laser (20 mW at 502 nm) at modulation frequency of 25 Hz. The performances of two PT methods are compared in the terms of their sensitivity, precision, reproducibility and limit of detection.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Prehrambena tehnologija

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