Naslov
Enkephalins in hematopoiesis

Autori
Boranić, Milivoj ; Križanac-Bengez, Ljiljana ; Gabrilovac, Jelka ; Marotti, Tanja ; Breljak, Davorka

Izvornik
Biomedicine & pharmacotherapy (0753-3322) 51 (1997), 1; 29-37

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
enkephalins; opioid peptides; hematopoiesis; immune response; CALLA/CD10; secondary messengers

Sažetak
Recent data support the view that neuropeptide mediators, in particular opioid peptides, participate in the control of hematopoiesis. The main arguments are: neuropeptides modulate the functions of lymphoid cells, macrophages and mature granulocytes ; they control cell proliferation and differentiation in many tissues, particularly during the embryogenesis ; lymphoid cells, macrophages, polymorphonuclear granulocytes and bone marrow stromal elements express neuropeptide receptors ; bone marrow cells produce opioid-like neuropeptides ; the CD10/CALLA marker of lymphoid, myeloid and marrow stromal cells is an enzyme, endopeptidase, which cleaves - and thus activates/inactivates - opioid and other neuropeptides. We have shown that opioid peptides enkephalins, opioid antagonist naloxone, and the inhibitor of enkephalin-degrading endopeptidase, thiorphan, modulated the proliferation and differentiation of hematopoietic cells in clonal and long-term cultures of mouse bone marrow. The effects partly depended on the presence of the accessory hematopoietic elements, and followed a circadian pattern. The dose-responses were irregular, showed strain-dependent and individual variations, and apparently reflected the state of the activity of target cells, cellular interactions, and simultaneous signals by other mediators. The enkephalins were shown to bind to specific (opioid) receptors on the target cells, and their signals to be transmitted to the cell interior by a cascade of secondary messengers including diacyl-glycerol (DAG), protein-kinase C (PKC) and CaČ++ ions. Neuropeptide regulation of hematopoiesis might belong to a complex immuno-neuroendocrine network including the melatonin.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA